Analysis of Asp aminoacyl synthetase.

Analysis performed on 2024-07-10 11:38:08

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 18095 145 17950 11303
Archaea 1091 22 1069 463
Vertebrata 803 199 604 434
Mammals 289 50 239 125

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
501 23 425

Domains

Domains
Current Study 1, 38
Guo et al (2010) 6, 24

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 464 37 501 12.540541
Number of Variants 409 39 448 10.487179
Number of Pathogenic Variants 22 1 23 22.000000
Number of Benign/Unknown Variants 387 38 425 10.184211
Fisher exact test 0.709312

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 482 19 501 25.368421
Number of Variants 426 22 448 19.363636
Number of Pathogenic Variants 23 0 23 Undefined
Number of Benign/Unknown Variants 403 22 425 18.318182
Fisher exact test 0.61834

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 464 37 0.95 (0.00) 0.74 (0.03) 9.914e-01 7.304e-01 15460.00 1.90e-16
Guo et al (2010) 482 19 0.94 (0.00) 0.70 (0.04) 9.912e-01 7.049e-01 8416.00 2.44e-10

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains